My Photo
Name:
Location: BACLIFF, Texas, United States

My mother was murdered by what I call corporate and political homicide i.e. FOR PROFIT! she died from a rare phenotype of CJD i.e. the Heidenhain Variant of Creutzfeldt Jakob Disease i.e. sporadic, simply meaning from unknown route and source. I have simply been trying to validate her death DOD 12/14/97 with the truth. There is a route, and there is a source. There are many here in the USA. WE must make CJD and all human TSE, of all age groups 'reportable' Nationally and Internationally, with a written CJD questionnaire asking real questions pertaining to route and source of this agent. Friendly fire has the potential to play a huge role in the continued transmission of this agent via the medical, dental, and surgical arena. We must not flounder any longer. ...TSS

Monday, June 16, 2014

Preclinical Detection of Variant CJD and BSE Prions in Blood

Preclinical Detection of Variant CJD and BSE Prions in Blood
 
Published: June 12, 2014 DOI: 10.1371/journal.ppat.1004202 Article About the Authors Metrics Comments Related Content Abstract Author Summary Introduction Methods Results Discussion Supporting Information Acknowledgments Author Contributions References Reader Comments (0) Media Coverage (3) Figures
 
Abstract
 
The emergence of variant Creutzfeldt Jakob Disease (vCJD) is considered a likely consequence of human dietary exposure to Bovine Spongiform Encephalopathy (BSE) agent. More recently, secondary vCJD cases were identified in patients transfused with blood products prepared from apparently healthy donors who later went on to develop the disease. As there is no validated assay for detection of vCJD/BSE infected individuals the prevalence of the disease in the population remains uncertain. In that context, the risk of vCJD blood borne transmission is considered as a serious concern by health authorities. In this study, appropriate conditions and substrates for highly efficient and specific in vitro amplification of vCJD/BSE agent using Protein Misfolding Cyclic Amplification (PMCA) were first identified. This showed that whatever the origin (species) of the vCJD/BSE agent, the ovine Q171 PrP substrates provided the best amplification performances. These results indicate that the homology of PrP amino-acid sequence between the seed and the substrate is not the crucial determinant of the vCJD agent propagation in vitro. The ability of this method to detect endogenous vCJD/BSE agent in the blood was then defined. In both sheep and primate models of the disease, the assay enabled the identification of infected individuals in the early preclinical stage of the incubation period. Finally, sample panels that included buffy coat from vCJD affected patients and healthy controls were tested blind. The assay identified three out of the four tested vCJD affected patients and no false positive was observed in 141 healthy controls. The negative results observed in one of the tested vCJD cases concurs with results reported by others using a different vCJD agent blood detection assay and raises the question of the potential absence of prionemia in certain patients.
 
Author Summary Variant Creutzfeldt Jakob Disease (vCJD) cases were identified in patients who received blood products that had been prepared from donors who later developed the disease. The blood borne transmission of vCJD is a major concern for blood transfusion banks, plasma derived products manufacturers and public health authorities. A vCJD blood screening test would represent an ideal solution for identifying donors/blood donations that might be at risk. In this study, we describe a blood assay which is based on the in vitro amplification of vCJD agent by Protein Misfolding Cyclic Amplification (PMCA). In vCJD animal models (sheep and primate), the assay enabled the identification of infected individuals in a very early stage of the asymptomatic incubation phase. We also provide evidence of the high specificity and the high analytical sensitivity of this assay using blood samples from vCJD affected and healthy patients.
 
 
snip...
 
Despite all the remaining difficulties, the results obtained so far by two different methodologies (PMCA as presented here and the abnormal PrP capture), and the rapid progress of QuIC derived technologies, allow potential new possibilities for vCJD screening and the prevention of its iatrogenic transmission.
 
 
 
 
Sunday, April 06, 2014
 
*** SPORADIC CJD and the potential for zoonotic transmission there from, either directly or indirectly via friendly fire iatrogenic mode, evidence to date
 
 
Wednesday, December 11, 2013
 
*** Detection of Infectivity in Blood of Persons with Variant and Sporadic Creutzfeldt-Jakob Disease ***
 
 
Monday, May 19, 2014
 
Variant CJD: 18 years of research and surveillance
 
 
Saturday, June 14, 2014
 
Rep. Rosa DeLauro (D-CT) Calls for Briefing on Beef Recalled for Mad Cow Potential Rep. Rosa DeLauro (D-CT)
 
 
Monday, June 9, 2014
 
TEXAS MAD COW COVER UP (human BSE) AGAIN IN TEXAS, Mr. President Sir, we need your help please
 
Governor Rick Perry has done everything he can to cover up mad cow disease and human TSE prion disease there from in Texas over the last 15 years or so. We have another nvCJD case here in Texas i.e. human BSE, still no information there from, another lame excuse, typical though, and more junk science, we need help Mr. President...
 
 
Thursday, June 12, 2014
 
*** Missouri Firm Recalls Ribeye and Carcass Products That May Contain Specified Risk Materials 4,012 pounds of fresh beef products because the dorsal root ganglia may not have been completely removed
 
 
Seven main threats for the future linked to prions
 
***Also, a link is suspected between atypical BSE and some apparently sporadic cases of Creutzfeldt-Jakob disease in humans. These atypical BSE cases constitute an unforeseen first threat that could sharply modify the European approach to prion diseases.
 
Second threat
 
snip...
 
 
Monday, October 10, 2011
 
EFSA Journal 2011 The European Response to BSE: A Success Story
 
snip...
 
*** but the possibility that a small proportion of human cases so far classified as "sporadic" CJD are of zoonotic origin could not be excluded. Moreover, transmission experiments to non-human primates suggest that some TSE agents in addition to Classical BSE prions in cattle (namely L-type Atypical BSE, Classical BSE in sheep, transmissible mink encephalopathy (TME) and chronic wasting disease (CWD) agents) might have zoonotic potential.
 
snip...
 
 
 
***In addition, non-human primates are specifically susceptible for atypical BSE as demonstrated by an approximately 50% shortened incubation time for L-type BSE as compared to C-type. Considering the current scientific information available, it cannot be assumed that these different BSE types pose the same human health risks as C-type BSE or that these risks are mitigated by the same protective measures.
 
 
***Infectivity in skeletal muscle of BASE-infected cattle
 
 
***feedstuffs- It also suggests a similar cause or source for atypical BSE in these countries.
 
 
Saturday, August 4, 2012
 
Final Feed Investigation Summary – California ATYPICAL L-TYPE BASE BSE Case - July 2012
 
 
PO-028: Oral transmission of L-type bovine spongiform encephalopathy (L-BSE) in primate model Microcebus murinus
 
 
***Also, a link is suspected between atypical BSE and some apparently sporadic cases of Creutzfeldt-Jakob disease in humans.
 
 
*** Creutzfeldt-Jakob Disease CJD cases rising North America with Canada seeing an extreme increase of 48% between 2008 and 2010 ***
 
 
Sunday, October 13, 2013
 
*** CJD TSE Prion Disease Cases in Texas by Year, 2003-2012
 
 
Tuesday, April 01, 2014
 
*** Questions linger in U.S. CJD cases 2005, and still do in 2014
 
 
Monday, June 9, 2014
 
*** TEXAS MAD COW COVER UP (human BSE) AGAIN IN TEXAS, Mr. President Sir, we need your help please
 
Governor Rick Perry has done everything he can to cover up mad cow disease and human TSE prion disease there from in Texas over the last 15 years or so. We have another nvCJD case here in Texas i.e. human BSE, still no information there from, another lame excuse, typical though, and more junk science, we need help Mr. President...
 
 
what’s the big secret about the age and history of this poor gentleman ???
 
MAD COW COVER UP USA, THE EVIDENCE MOUNTS $$$
 
Monday, June 02, 2014
 
*** Confirmed Human BSE aka mad cow Variant CJD vCJD or nvCJD Case in Texas
 
 
 
 
Friday, April 25, 2014
 
Accuracy of administrative diagnostic data for pathologically confirmed cases of Creutzfeldt-Jakob disease
 
Article in Press
 
 
 
TSS

0 Comments:

Post a Comment

Subscribe to Post Comments [Atom]

Links to this post:

Create a Link

<< Home